NASC

NASC ID: N849978

Name: WiscDsLox259A10

ABRC stock number: CS849978

Description: Sequence-indexed T-DNA insertion line; the T-DNA includes a Ds transposon and a LoxP recombination site. A Basta resistance marker is used to select for the T-DNA and for the Ds transposon following any transposition. However, be aware that lines from this set might not be able to launch their Ds element. Be sure to test any line for the ability to transpose before using it in experiments. Hygromycin resistance can be used for selection of lines where the Ds element has been excised by transposition. WiscDsLox lines do not contain Ac transposase, and the lines should be crossed to a line expressing Ac transposase in order to mobilize the Ds transposon. May be segregating for phenotypes that are not linked to the insertion. May have additional insertions potentially segregating.

Associated Polymorphisms:

Wiscseq_DsLox259A10.2 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G30800.1 (chr 2 pos 13124353 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG797018.

Wiscseq_DsLox259A10.3 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G44530.2-UTR3 and AT3G44540.1-P355 (chr 3 pos 16122820 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank ID: KG797020.

Wiscseq_DsLox259A10.4 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G58190.1-P355 and AT3G58193-P355 (chr 3 pos 21553113 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG797021.

Wiscseq_DsLox259A10.5 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G15355.1 (chr 3 pos 5186195 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG797019.

Donation Date: 2004-12-01

Donated by:

University of Wisconsin, Madison Patrick Krysan University of Wisconsin, Madison Richard Amasino University of Wisconsin, Madison Michael Sussman

Donor Number: WiscDsLox259A10

Part of : Wisconsin DsLox T-DNA lines

Stock type: individual line

Material type: seed

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: T-DNA Insertion

Background: Col-2

Transformation method: Agrobacterium